Journal: PLOS One

Article Title: A bioactive hydrogel integrating bFGF/VEGFA gene-loaded nanoparticles and platelet-rich plasma for accelerated full-thickness skin wound healing

doi: 10.1371/journal.pone.0350087

Figure Lengend Snippet: (A) Representative Hematoxylin & eosin (H&E) staining images of wound sections; The red arrow refers to the skin appendage. (B) Representative Masson’s trichrome staining images of wound sections; blue color indicates collagen. (C) Representative immunohistochemical images of wound sections showing type-I collagen (COLI) expression. (D) Representative immunohistochemical images of wound sections showing type-III collagen (COLIII) expression. (E) Quantification of total collagen deposition (Masson-stained area %). (F) Quantification of COLI deposition (% positively stained area). (G) Quantification of COLIII deposition (% positively stained area). In E–G, data are mean ± SD from three independent animals (n = 3 mice per group). Statistical significance was evaluated by one-way ANOVA and LSD comparison test. Ns, no significant difference (P > 0.05); *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Anti-bFGF antibody (bsm-34016R, Bioss, China, dilution 1:250), anti-VEGFA antibody (ab46154, Abcam, UK, dilution 1:250), anti-CD 31 antibody (28083–1-AP, Proteintech, USA, dilution 1:250), anti-Ki 67 antibody (ab209897, Abcam, UK, dilution 1:250), anti-Bcl 2 antibody (26593–1-AP, Proteintech, USA, dilution 1:250), anti-Collagen Type I antibody(HY- P81227 , MCE, USA, dilution 1:250), anti- Collagen Type III antibodies (HY- P81101 , MCE, USA, dilution 1:250) were added to the slices and incubated overnight.

Techniques: Staining, Immunohistochemical staining, Expressing, Comparison